An R package for the analysis and result reporting of RNA-Seq data by combining multiple statistical algorithms


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Documentation for package ‘metaseqR2’ version 1.21.0

Help Pages

buildAnnotationDatabase Build a local annotation database for metaseqR2
buildCustomAnnotation Import custom annotation to the metaseqR2 annotation database from GTF file
combineBonferroni Combine p-values with Bonferroni's method
combineHarmonic Combine p-values using weights
combineMaxp Combine p-values using the maximum p-value
combineMinp Combine p-values using the minimum p-value
combineSimes Combine p-values with Simes' method
combineWeight Combine p-values using weights
createSignalTracks Create bigWig signal tracks
diagplotAvgFtd Create average False (or True) Discovery curves
diagplotBoxplot Boxplots wrapper for the metaseqR2 package
diagplotCor Summarized correlation plots
diagplotDeHeatmap Diagnostic heatmap of differentially expressed genes
diagplotEdaseq Diagnostic plots based on the EDASeq package
diagplotFiltered Diagnostic plot for filtered genes
diagplotFtd Create False (or True) Positive (or Negative) curves
diagplotMds Multi-Dimensinal Scale plots or RNA-Seq samples
diagplotNoiseq Diagnostic plots based on the NOISeq package
diagplotPairs Massive X-Y, M-D correlation plots
diagplotRoc Create basic ROC curves
diagplotVenn Venn diagrams when performing meta-analysis
diagplotVolcano (Interactive) volcano plots of differentially expressed genes
downsampleCounts Downsample read counts
estimateAufcWeights Estimate AUFC weights
estimateSimParams Estimate negative binomial parameters from real data
getAnnotation Annotation downloader
getDefaults Default parameters for several metaseqr functions
getInstalledAnnotations Load a metaseqR2 annotation element
getWeights Get precalculated statistical test weights
hg19pvalues p-values from human RNA-Seq data with two conditions, four samples
importCustomAnnotation Import a metaseqR2 custom annotation element
libsizeListMm9 Mouse RNA-Seq data with two conditions, four samples
loadAnnotation Load a metaseqR2 annotation element
makeSimDataSd Create simulated counts using the Soneson-Delorenzi method
makeSimDataTcc Create simulated counts using TCC package
metaseqr2 The main metaseqr2 pipeline
metaseqrPlot Diagnostic plots for the metaseqR2 package
metaTest Meta-analysis using several RNA-Seq statistics
mm9GeneCounts Mouse RNA-Seq data with two conditions, four samples
normalizeAbsseq Normalization based on the ABSSeq package
normalizeDeseq Normalization based on the DESeq package
normalizeDeseq2 Normalization based on the DESeq2 package
normalizeDss Normalization based on the DSS package
normalizeEdaseq Normalization based on the EDASeq package
normalizeEdger Normalization based on the edgeR package
normalizeNbpseq Normalization based on the NBPSeq package
normalizeNoiseq Normalization based on the NOISeq package
read2count SAM/BAM/BED file reader helper for the metaseqr2 pipeline
readTargets Creates sample list and BAM/BED file list from file
sampleListMm9 Mouse RNA-Seq data with two conditions, four samples
statAbsseq Statistical testing with ABSSeq
statDeseq Statistical testing with DESeq
statDeseq2 Statistical testing with DESeq2
statDss Statistical testing with DSS
statEdger Statistical testing with edgeR
statLimma Statistical testing with limma
statNbpseq Statistical testing with NBPSeq
statNoiseq Statistical testing with NOISeq