# Annotation and analysis of biological dynamical systems using flow cytometry

## Documentation for package ‘flowTime’ version 1.33.0

- DESCRIPTION file.
- User guides, package vignettes and other documentation.
- Package NEWS.

## Help Pages

addbs | Add background subtraction to a summary data frame
addnorm | Normalize fluorescence
annotateFlowSet | Annotate a flowSet with experimental metadata
createAnnotation | Create an annotation dataframe
dipdoubletGate | A gate for the set of all diploid doublets
dipsingletGate | A gate for the set of all diploid singlet yeast cells
flsummary | Get summary statistics for fluorescence or other data channels of a flowSet
getTime | Get the time at which at flowFrame began collection
hapdoubletGate | A gate for the set of all haploid doublets
hapsingletGate | A gate for the set of all haploid singlets
loadGates | Load a yeast gate file
meanMedianSD | Summary statistic columns for a flow frame
ploidy | Guess the ploidy of a given flowframe
polyGate | Create a polygon gate
qaGating | Quality assurance check
read.plateSet | Read FCS files from set of plates
saveGates | Save a yeast gate set
steadyState | Analysis of steady state fluorescence flow cytometry
summarizeFlow | Generate summary statistics for a flowSet
tidyFlow | Generate a tidy dataset from time-course flow cytometry data
yeastGate | A gate for the set of all yeast cells